Intermediate

Part:BBa_K112343:Design

Designed by: Aron Lau   Group: iGEM08_UC_Berkeley   (2008-10-28)


{rbs_lamB>} {< r.BglII>}{< phoA!}{< b1006>}


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 977
    Illegal NgoMIV site found at 1424
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Digest (BgIII methylated) K112331 AC     (BamHI/XhoI)
Digest (BamHI methylated) K112324 CK     (BgIII/XHoI)
Product is pBjh1601AK-K112343


Source

BBa_K112331 and BBa_K112324


References